Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human & Mouse LC3B II Detection Kit, 500 Assay Points
HTRF Human & Mouse LC3B II Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total Cyclin D1 Detection Kit, Control Lysate
HTRF Human & Mouse Total Cyclin D1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 10,000 Assay Points
Ace Glass, Inc. 100mL vessel with sloped shoulders and a #25 Ace-Thred neck
100mL vessel with sloped shoulders and a #25 Ace-Thred neck; includes PTFE lined cap. For use in freeze drying and storage. After drying material, vessel can be capped and stored. Working capacity of vessel is about 1/2 of volume.
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Protein Disaggregation Detection Kit, 100 samples
HTRF Protein Disaggregation Detection Kit, 100 samples
Molecular Devices Filter Lens, 600 nm, Microplate compatible, Replacement
Replacement filter lens for microplate and ELISA readers. This optical component provides centered transmission at 600 nm and is sold as a spare replacement for compatible plate readers including EMax, VMax, UVMax, and ThermoMax models.
- Provides centered transmission at 600 nm for absorbance measurements.
- Designed for use in microplate and ELISA reader detection optics.
- Supplied as a single replacement lens for instrument maintenance.
- Helps maintain assay accuracy for absorbance-based protocols.
Molecular Devices Polarization Emission Filter, Visible, Pair, For FilterMax Readers
Replacement polarization emission filter set for fluorescence polarization measurements in multi-mode microplate readers. Supplied as a pair (quantity 2), these emission filters are intended to enable polarization-based fluorescence detection and improve signal discrimination in applicable assays.
- Replacement emission filters for fluorescence polarization assays.
- Visible emission band, vendor designation "Visible".
- Sold as a pair (quantity 2) for filter-based reader configurations.
- Intended for compatible multi-mode microplate readers.
- Helps improve polarization signal discrimination and assay reproducibility.
Molecular Devices Absorbance Filter, 515 nm, 8 nm
Absorbance bandpass filter suitable for microplate absorbance assays. Centered at 515 nm with an 8 nm bandwidth, this optical filter installs in filter slide slots and provides narrowband absorbance detection for plate-based assays. Compatible with FilterMax F3 and F5 multimode microplate readers.
- Center wavelength 515 nm.
- Bandwidth 8 nm.
- Filter type Absorbance bandpass.
- Application Microplate absorbance measurements.
- Form factor Slide-mounted filter for filter-based readers.